Weiss A, Sommer P, Wilbertz JH. Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons. J Vis Exp. 2023 Jul 7;(197). doi: 10.3791/65570. PMID: 37486112 (read article here).
In this article and video, we describe a protocol to obtain and analyze phenotypic profiles from commercially available human mDA neurons using clustering or machine learning-driven supervised classification methods. The described protocol will particularly interest researchers working on neuronal disease modeling or studying chemical compound effects in human neurons.
This protocol was used in our study on Parkinson’s disease published in Stem Cell reports in September 2022 (read news here).
